Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions
نویسندگان
چکیده
KISCOi001-A is a healthy feeder-free and fully characterized human induced pluripotent stem (iPS) cell line cultured under xeno-free defined conditions. The generated from normal foreskin fibroblasts with non-integrating episomal plasmid vectors encoding OCT4, SOX2, KLF4, NANOG, LIN28, nontransforming L-MYC dominant negative p53. iPS cells are transgene-free their pluripotency confirmed by the expression of markers capacity to differentiate into ectoderm, endoderm mesoderm while identity karyotype stability Genomic assays.
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ژورنال
عنوان ژورنال: Stem Cell Research
سال: 2021
ISSN: ['1876-7753', '1873-5061']
DOI: https://doi.org/10.1016/j.scr.2021.102193